Vibrio cholerae HlyA hemolysin is processed by proteolysis
نویسندگان
چکیده
منابع مشابه
Hemolysin-destructive factor of Vibrio cholerae (Vibrio comma).
Feeley and Pittman (Bull. World Health Organ. 28:347, 1963) speculated that a hemolysin (HL) destructive enzyme produced by El Tor vibrio at a late stage of growth lowers the HL titer. We proved the production of an HL-destructive factor (HDF) by strains of classical cholera and El Tor vibrio and established an in vitro method for quantitative titration of HDF. HL was prepared by growing a hemo...
متن کاملCytotoxic cell vacuolating activity from Vibrio cholerae hemolysin.
A Vibrio cholerae cytotoxin, designated VcVac, was found to cause vacuolation in Vero cells. It was originally detected in the pathogenic O1 Amazonia variant of V. cholerae and later shown to be produced in environmental strains and some El Tor strains. Comparison of VcVac production in various strains suggested that hemolysin was responsible for the vacuolating phenotype. Genetic experiments e...
متن کاملHemolysin production and cloning of two hemolysin determinants from classical Vibrio cholerae.
The hemolytic activity of 20 classical and 3 El Tor strains of V. cholerae O1 was examined phenotypically and genetically. The El Tor strains lysed bovine, chicken, human, rabbit, and sheep erythrocytes (RBCs), while the classical strains lysed only chicken and rabbit RBCs. The assay was done with RBCs in Tris-NaCl buffer, since phosphate-buffered saline was found to inhibit hemolytic activity....
متن کاملVibrio cholerae hemolysin: The β-trefoil domain is required for folding to the native conformation
Vibrio cholerae cytolysin/hemolysin (VCC) is a 65 kDa β-pore-forming toxin causing lysis and death of eukaryotic cells. Apart from the core cytolysin domain, VCC has two lectin domains with β-trefoil and β-prism folds. The β-prism domain binds to cell surface carbohydrate receptors; the role of the β-trefoil domain is unknown. Here, we show that the pro-VCC mutant without the β-trefoil domain f...
متن کاملVibrio cholerae Hemolysin Is Required for Lethality, Developmental Delay, and Intestinal Vacuolation in Caenorhabditis elegans
BACKGROUND Cholera toxin (CT) and toxin-co-regulated pili (TCP) are the major virulence factors of Vibrio cholerae O1 and O139 strains that contribute to the pathogenesis of disease during devastating cholera pandemics. However, CT and TCP negative V. cholerae strains are still able to cause severe diarrheal disease in humans through mechanisms that are not well understood. METHODOLOGY/PRINCI...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1990
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.58.10.3375-3379.1990